Coding

Part:BBa_K1114411:Experience

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-07)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1114411

This is the MoClo formatted part of lacZ Alpha with EB fusion sites. This is a Level 0 MoClo part with flanking sites E on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See pSB1C3.

User Reviews

UNIQ50361def0a3df882-partinfo-00000001-QINU UNIQ50361def0a3df882-partinfo-00000002-QINU